<Department of Microbiology & Immunology, UT Health Science Center at San Antonio Double Helix rotating/>

Synthesis


Oligo Request Form         Online Request Form        Large Order Request Form         FAQs

The NACF provides low cost custom DNA synthesis with unparalleled turnaround times to investigators at the HSC and surrounding scientific community.

Prices listed start May 1, 2007


Our oligos are synthesized on an ABI 3900 or a BioAutomation MerMade-6 synthesizer using beta-cyanoethyl chemistry. Synthesis begins with the 3' terminal nucleoside that is attached to a solid support, and the remaining nucleotide bases are added one at a time until the synthesis is complete.


Features

    •   Deprotected, Lyophilized, and Ready for Use
    •   Fast, Same or Next Day Turnaround Time (*Please see details below.)
    •   Purification Upon Request

Service

Scale

 

Price

 

Guaranteed Minimum OD260

 

Approximate Yield

 

Primer

40 nmole

$0.34 / base

50 bases =3 OD260

5-35 nmol

Standard

200 nmole

$0.95 / base

10 OD260

40-150 nmol

Bulk

1 μmole

$2.25 / base

50 OD260

200-800 nmol


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Chemical reactions in the synthesis cycle are not flawless, rather 98-99% efficient. With each base addition, small amounts of sequences fail to undergo a base addition or coupling. Most of these failed sequences are chemically capped during the synthesis cycle preventing them from participating in further base additions. However, because efficiency is not 100% some sequences will remain uncapped and are available for further base additions, resulting in n-1-mer, n-2-mer, etc. failure sequences. Reverse phase cartridge purification allows for the separation of the full-length product from these failure sequences.

Oligo Purification Services

Type

Scale

 

Price

(Add on to per/base price)

 

 

Turnaround Time

 

 

Recommended Applications

 

Desalt-

Removal of trace amounts of organic synthesis by-products.

Any

$2.00 /
oligo

Next Day

antisense, oligo probes,
hybridization, RT-PCR

Reverse Phase Cartridge-

Removal of synthesis by-products and non-full-length products. Results in 95%-99% full-length product.

40 nmol

$12.00 / oligo

1-3 Days

cloning, end-labeling, gel shift assay, gene synthesis, kinasing, mutagenesis

200 nmol

$18.20 / oligo

1-3 Days

1 μmol

$25.00 / oligo

1-3 Days

Purification Considerations:

Yield is length-dependent for RPC purification.

Maximum allowable length for RPC purification is 25 bases for the 40 nmol scale and 50 bases for the 200 nmol and 1 μmol scales.

 

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Synthesis of specialty oligos with minor bases or labels is available through the purchase of the specialty reagent. Once diluted, the shelf life of most of these reagents is 1-2 days making it necessary to charge for the entire bottle. One bottle of reagent is usually sufficient for the synthesis of multiple labeled oligos depending on the synthesis scale (detailed information below), so plan ahead for cost-effectiveness.

Labeling Reagents & Specialty Bases

Offered by the Bottle

Label

Bottle Size

Number of Additions Per Scale

Price Per Bottle

40 nmol

200 nmol

1μmol

Biotin

5' end-label

50 μmol

20

13

5

$100

100 μmol

45

30

12

$180

Fluorescein

(6-FAM)

5' end-label

50 μmol

20

13

5

$132

100 μmol

45

30

12

$260

Phosphate
5' end-label

100 μmol

45

30

12

$40

Phosphate II
5' end-label
(compatible w/ purification)

100 μmol

45

30

12

$48

Inosine

100 μmol

45

30

12

$40

Uracil

100 μmol

45

30

12

$40

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Standard Ordering

For your convenience and the fastest turnaround, we offer several options of ordering directly from our lab. No PeopleSoft requisition is required when using any standard ordering method.


By Synthesis Request Form

Typed information is preferred, but forms may also be printed and completed by hand. Completed forms may be hand-carried, e-mailed*, or faxed to the Core Facility. After normal operating hours, forms may be left in the Oligo Drop Box located outside the lab.

To type a form, use the Online Oligo Request Form and either print it or email it as an attachment; to complete a form by hand, use the Oligo Request Form.


*Note: By emailing forms you are consenting for charges to be made to the indicated account and also verifying that you are authorized on the account.

By Email Without a Form (1-9 oligos)

Authorized users on an account may email oligo requests to oligos@uthscsa.edu. Please use the following guidelines:

  1. Include all of the following information:
    • USER NAME (first and last name)
    • PI NAME
    • CONTACT PHONE NUMBER
    • ACCOUNT NUMBER
    • DEPARTMENT
  2. Use all CAPS for oligo sequences.
  3. Do not use spaces in oligo sequences.
  4. Indicate scale and level of purification for each oligo.
* Note: By emailing an order you are consenting for charges to be made to the indicated account and also verifying that you are authorized on the account.

By Large Order Request Form (10+ oligos)
Complete the Large Order Request Form and email it to oligos@uthscsa.edu.


General Stores Ordering

Order through Stores using PeopleSoft and the Stores website.

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If standard ordering was used, you will be notified by the NACF staff by phone or email as soon as your order is ready for pick-up. Off-campus users must include shipping information if orders are to be sent directly to off-campus locations. When an order is placed through General Stores, General Stores will contact you by phone when the order is ready. Off-campus orders made through General Stores will be delivered following Stores' policies.

 

Turnaround Times

 

 

Expected Turnaround

 

 

Scale

 

Details

Same Day

40 nmol-200 nmol

must be received by 9:30am,
10-30 bases,
non-RPC purified, non-desalted,
labeled/unlabeled,
no large orders (10 or more oligos)

Next Day

40 nmol-200 nmol

10-100+ bases,
non-RPC purified,
may be desalted,
labeled/unlabeled

1-2 days

40 nmol-200 nmol

large orders (10 or more oligos), non-RPC purified

1-2 days

1 μmol

non-RPC purified

1-3 days

40 nmol - 1 μmol

RPC purified

*Important Note: Synthesis services are offered on a first come, first serve basis. This means that when our daily maximum run capacity is reached, oligos will be in queue to be processed. Our maximum run capacity limits us to 45 same day primers, which must be received by the NACF by 9:30am. Every effort is made to maintain quick turnaround times, but unfortunately we cannot guarantee them. Users will be notified at the time of submission or promptly afterward if an expected turnaround time is not going to be met. Off-campus users using campus mail may expect a delayed turnaround time.

 

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